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A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost


Reference:

Williams, J., Clarkson, J. M., Mills, P. R. and Cooper, R. M., 2003. A selective medium for quantitative reisolation of Trichoderma harzianum from Agaricus bisporus compost. Applied and Environmental Microbiology, 69 (7), pp. 4190-4191.

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http://dx.doi.org/10.1128/AEM.69.7.4190-4191.2003

Abstract

We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.

Details

Item Type Articles
CreatorsWilliams, J., Clarkson, J. M., Mills, P. R. and Cooper, R. M.
DOI10.1128/AEM.69.7.4190-4191.2003
DepartmentsFaculty of Science > Biology & Biochemistry
RefereedYes
StatusPublished
ID Code4021
Additional InformationID number: ISI:000184082100066

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