Identification of centaurin-alpha 2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle
Whitley, P., Gibbard, A. M., Koumanov, F., Oldfield, S., Kilgour, E. E., Prestwich, G. D. and Holman, G. D., 2002. Identification of centaurin-alpha 2: a phosphatidylinositide-binding protein present in fat, heart and skeletal muscle. European Journal of Cell Biology, 81 (4), pp. 222-230.
Related documents:This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below.
We describe here the cloning, expression and characterisation of centaurin-alpha2 from a rat adipocyte cDNA library. The centaurin-alpha2 cDNA contains an open reading frame, which codes for a protein of 376 amino acids with predicted mass of 43.5 kDa. Centaurin-alpha2 shares 51-59% identity with centaurin-alpha1 proteins and has the same domain organisation, consisting of a predicted N-terminal ArfGAP domain followed by two successive pleckstrin homology domains. Despite the sequence similarity, there are a number of notable differences between the previously characterised centaurin-alpha1 proteins and the newly described centaurin-alpha2: (i) in vitro lipid binding experiments with centaurin-alpha2 do not reveal the same selectivity for phosphatidylinositol 3,4,5-trisphosphate over phosphatidylinositol 4,5-bisphosphate that has been shown for centaurin-alpha1; (H) unlike centaurin-alpha1 which is expressed mainly in the brain, centaurin-alpha2 has a broad tissue distribution, being particularly abundant in fat, heart and skeletal muscle; (iii) in contrast to centaurin-alpha1 which is found in both membrane and cytosolic fractions, endogenous centaurin-alpha2 is exclusively present in the dense membrane fractions of cell extracts, suggesting a constitutive membrane association. Insulin stimulation, which stimulates phosphatidylinositol 3,4,5-trisphosphate production, does not alter the subcellular distribution of centaurin-alpha2 between adipocyte membrane fractions. This observation is consistent with the lack of specificity of centaurin-alpha2 for phosphatidylinositol 3,4,5-trisphosphate over phosphatidylinositol 4,5-bisphosphate.
|Creators||Whitley, P., Gibbard, A. M., Koumanov, F., Oldfield, S., Kilgour, E. E., Prestwich, G. D. and Holman, G. D.|
|Departments||Faculty of Science > Biology & Biochemistry|
Actions (login required)