Rhodothermus marinus: a thermophilic bacterium producing dimeric and hexameric citrate synthase isoenzymes
Karlsson, E. N., Abou-Hachem, M., Holst, O., Danson, M. J. and Hough, D. W., 2002. Rhodothermus marinus: a thermophilic bacterium producing dimeric and hexameric citrate synthase isoenzymes. Extremophiles, 6 (1), pp. 51-56.
Related documents:This repository does not currently have the full-text of this item.
You may be able to access a copy if URLs are provided below.
Two separate citrate synthases from the extremely thermophilic bacterium Rhodothermus marinus have been identified and purified. One of the enzymes is a hexameric protein and is the first thermostable, hexameric citrate synthase to be isolated. The other is a dimeric enzyme, which is also thermostable but possesses both citrate synthase and 2-methyl citrate synthase activities. 2-Methyl citrate synthase uses propionyl-coenzyme A as one of its substrates and in Escherichia coli, for example, it has been implicated in the metabolism of propionate. However, no growth of R. marinus was observed using minimal medium with propionate as the sole carbon source, and both hexameric and dimeric enzymes were produced irrespective of whether propionate was included in the growth medium. The data are discussed with respect to the evolutionary relationships between the known hexameric and dimeric citrate synthases and 2-methyl citrate synthase.
|Creators||Karlsson, E. N., Abou-Hachem, M., Holst, O., Danson, M. J. and Hough, D. W.|
|Departments||Faculty of Science > Biology & Biochemistry|
|Additional Information||ID number: ISI:000173961500007|
Actions (login required)