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Protein phosphorylation detection using dual-mode field-effect devices and nanoplasmonic sensors


Reference:

Bhalla, N., Di Lorenzo, M., Pula, G. and Estrela, P., 2015. Protein phosphorylation detection using dual-mode field-effect devices and nanoplasmonic sensors. Scientific Reports, 5, 8687.

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    Official URL:

    http://dx.doi.org/10.1038/srep08687

    Abstract

    Phosphorylation by kinases is an important post-translational modification of proteins. It is a critical control for the regulation of vital cellular activities, and its dysregulation is implicated in several diseases. A common drug discovery approach involves, therefore, time-consuming screenings of large libraries of candidate compounds to identify novel inhibitors of protein kinases. In this work, we propose a novel method that combines localized surface plasmon resonance (LSPR) and electrolyte insulator semiconductor (EIS)-based proton detection for the rapid identification of novel protein kinase inhibitors. In particular, the selective detection of thiophosphorylated proteins by LSPR is achieved by changing their resonance properties via a pre-binding with gold nanoparticles. In parallel, the EIS field-effect structure allows the real-time electrochemical monitoring of the protein phosphorylation by detecting the release of protons associated with the kinases activity. This innovative combination of both field-effect and nanoplasmonic sensing makes the detection of protein phosphorylation more reliable and effective. As a result, the screening of protein kinase inhibitors becomes more rapid, sensitive, robust and cost-effective.

    Details

    Item Type Articles
    CreatorsBhalla, N., Di Lorenzo, M., Pula, G. and Estrela, P.
    DOI10.1038/srep08687
    DepartmentsFaculty of Engineering & Design > Electronic & Electrical Engineering
    Faculty of Engineering & Design > Chemical Engineering
    Faculty of Science > Pharmacy & Pharmacology
    Research CentresCentre for Advanced Sensor Technologies (CAST)
    Centre for Sustainable Chemical Technologies
    Centre for Regenerative Medicine
    RefereedYes
    StatusPublished
    ID Code43222

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