Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum

Reference:

Marshall, D., Soliakov, L., Redfern, P. and Wonnacott, S., 1996. Tetrodotoxin-sensitivity of nicotine-evoked dopamine release from rat striatum. Neuropharmacology, 35 (11), pp. 1531-1536.

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Official URL:

http://dx.doi.org/10.1016/S0028-3908(96)00117-7

Abstract

Recent observations from synaptosome preparations have questioned the tetrodotoxin (TTX) insensitivity of nicotine-evoked dopamine release in the striatum, a characteristic previously considered diagnostic of presynaptically located nicotinic acetylcholine receptors (nAChRs). Therefore, we have undertaken a comparison of nicotine-evoked dopamine release in the presence of TTX from the rat striatum in vitro, using synaptosomes and brain slices, and in vivo, using microdialysis. In P2 and Percoll-purified synaptosome preparations, 1.5 mu M TTX partially inhibited nicotine-evoked [H-3]dopamine release by 54% and 37%, respectively, whereas in more intact preparations (brain slices and microdialysis) TTX completely inhibited mecamylamine-sensitive nicotine-stimulated dopamine release. These results suggest that caution should be exercised in the interpretation of TTX sensitivity of nicotine-evoked responses with regard to the location of nAChRs.

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