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Attachment and phospholipase A(2)-induced lysis of phospholipid bilayer vesicles to plasma-polymerized maleic anhydride/SiO2 multilayers


Reference:

Chifen, A. N., Forch, R., Knoll, W., Cameron, P. J., Khor, H. L., Williams, T. L. and Jenkins, A. T. A., 2007. Attachment and phospholipase A(2)-induced lysis of phospholipid bilayer vesicles to plasma-polymerized maleic anhydride/SiO2 multilayers. Langmuir, 23 (11), pp. 6294-6298.

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Abstract

This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydride films covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) combined with surface plasmon resonance spectroscopy (SPR) was used to monitor the activation of plasma-deposited maleic anhydride (pp-MA) film with EDC/NHS and the subsequent coupling of lipid vesicles. The vesicles were formed from a mixture of phosphatidylcholine and phosphatidylethanolamine lipids, with a water-soluble fluorophore encapsulated within. Vesicle attachment was measured in real time on plasma films formed under different pulse conditions (plasma duty cycle). Optimum vesicle attachment was observed on the pp-MA films containing the highest density of maleic anhydride groups. Phospholipase A(2) was used to lyse the surface-bound vesicles and to release the encapsulated fluorophore.

Details

Item Type Articles
CreatorsChifen, A. N., Forch, R., Knoll, W., Cameron, P. J., Khor, H. L., Williams, T. L. and Jenkins, A. T. A.
DOI10.1021/la0701273
DepartmentsFaculty of Science > Chemistry
RefereedYes
StatusPublished
ID Code4619
Additional InformationID number: ISI:000246456700064

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