Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes
Reference:
Williams, T. L., Vareiro, M. and Jenkins, A. T. A., 2006. Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes. Langmuir, 22 (15), pp. 6473-6476.
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Abstract
This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.
Details
| Item Type | Articles |
| Creators | Williams, T. L., Vareiro, M. and Jenkins, A. T. A. |
| DOI | 10.1021/la060853q |
| Departments | Faculty of Science > Chemistry |
| Refereed | Yes |
| Status | Published |
| ID Code | 4642 |
| Additional Information | ID number: ISI:000238926000004 |
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