Research

Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes


Reference:

Williams, T. L., Vareiro, M. and Jenkins, A. T. A., 2006. Fluorophore-encapsulated solid-supported bilayer vesicles: A method for studying membrane permeation processes. Langmuir, 22 (15), pp. 6473-6476.

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Abstract

This letter describes a new method for studying the interaction of the membrane-lysing enzyme phospholipase A(2) (PLA(2)) with phospholipid bilayers by simultaneous measurements of enzyme binding and vesicle lysis using surface plasmon resonance (SPR) and permeabilization using surface plasmon field-enhanced fluorescence spectroscopy (SPFS). The PLA(2) inhibitor dimethyl-eicosadienoic acid was incorporated into the surface-bound vesicles and support bilayer in order to study its role in preventing PLA(2)-mediated vesicle lysis. This methodology has a generic applicability for the study of a range of membrane-disrupting agents.

Details

Item Type Articles
CreatorsWilliams, T. L., Vareiro, M. and Jenkins, A. T. A.
DOI10.1021/la060853q
DepartmentsFaculty of Science > Chemistry
RefereedYes
StatusPublished
ID Code4642
Additional InformationID number: ISI:000238926000004

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