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Ecological overlap and horizontal gene transfer in Staphylococcus aureus and Staphylococcus epidermidis


Reference:

Méric, G., Miragaia, M., de Been, M., Yahara, K., Pascoe, B., Mageiros, L., Mikhail, J., Harris, L. G., Wilkinson, T. S., Rolo, J., Lamble, S., Bray, J. E., Jolley, K. A., Hanage, W. P., Bowden, R., Maiden, M. C. J., Mack, D., de Lencastre, H., Feil, E. J., Corander, J. and Sheppard, S. K., 2015. Ecological overlap and horizontal gene transfer in Staphylococcus aureus and Staphylococcus epidermidis. Genome Biology and Evolution, 7 (5), pp. 1313-1328.

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http://dx.doi.org/10.1093/gbe/evv066

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Abstract

The opportunistic pathogens Staphylococcus aureus and Staphylococcus epidermidis represent major causes of severe nosocomial infection, and are associated with high levels of mortality and morbidity worldwide. These species are both common commensals on the human skin and in the nasal pharynx, but are genetically distinct, differing at 24% average nucleotide divergence in 1,478 core genes. To better understand the genome dynamics of these ecologically similar staphylococcal species, we carried out a comparative analysis of 324 S. aureus and S. epidermidis genomes, including 83 novel S. epidermidis sequences. A reference pan-genome approach and whole genome multilocus-sequence typing revealed that around half of the genome was shared between the species. Based on a BratNextGen analysis, homologous recombination was found to have impacted on 40% of the core genes in S. epidermidis, but on only 24% of the core genes in S. aureus. Homologous recombination between the species is rare, with a maximum of nine gene alleles shared between any two S. epidermidis and S. aureus isolates. In contrast, there was considerable interspecies admixture of mobile elements, in particular genes associated with the SaPIn1 pathogenicity island, metal detoxification, and the methicillin-resistance island SCCmec. Our data and analysis provide a context for considering the nature of recombinational boundaries between S. aureus and S. epidermidis and, the selective forces that influence realized recombination between these species.

Details

Item Type Articles
CreatorsMéric, G., Miragaia, M., de Been, M., Yahara, K., Pascoe, B., Mageiros, L., Mikhail, J., Harris, L. G., Wilkinson, T. S., Rolo, J., Lamble, S., Bray, J. E., Jolley, K. A., Hanage, W. P., Bowden, R., Maiden, M. C. J., Mack, D., de Lencastre, H., Feil, E. J., Corander, J. and Sheppard, S. K.
DOI10.1093/gbe/evv066
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URLURL Type
http://www.scopus.com/inward/record.url?scp=84942321996&partnerID=8YFLogxKUNSPECIFIED
Uncontrolled Keywordsecological and environmental processes,evolution, molecular,gene transfer, horizontal,genes, fungal,genetic variation,genome, fungal,homologous recombination,staphylococcus aureus,staphylococcus epidermidis
DepartmentsFaculty of Science > Biology & Biochemistry
Research CentresCentre for Mathematical Biology
EPSRC Centre for Doctoral Training in Statistical Mathematics (SAMBa)
Milner Centre for Evolution
RefereedYes
StatusPublished
ID Code47657
Additional Information© The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

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