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The red edge excitation shift phenomenon can be used to unmask protein structural ensembles:implications for NEMO-ubiquitin interactions


Reference:

Catici, D. A. M., Amos, H. E., Yang, Y., van den Elsen, J. M. H. and Pudney, C. R., 2016. The red edge excitation shift phenomenon can be used to unmask protein structural ensembles:implications for NEMO-ubiquitin interactions. FEBS Journal, 283 (12), pp. 2272-2284.

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    Official URL:

    http://dx.doi.org/10.1111/febs.13724

    Abstract

    To understand complex molecular interactions it is necessary to account for molecular flexibility and the available equilibrium of conformational states. Only a small number of experimental approaches can access such information. Potentially steady-state red edge excitation shift (REES) spectroscopy can act as a qualitative metric of changes to the protein free energy landscape (FEL) and the equilibrium of conformational states. First we validate this hypothesis using a single Trp containing protein, NF-κB essential modulator (NEMO). We provide detailed evidence from chemical denaturation studies, macromolecular crowding studies and the first report of the pressure-dependence of the REES effect. Combined these data demonstrate that the REES effect can report on the 'ruggedness' of the FEL and we present a phenomenological model, based on realistic physical interpretations, for fitting steady-state REES data to allow quantification of this aspect of the REES effect. We test the conceptual framework we have developed by correlating findings from NEMO ligand binding studies with the REES data in a range of NEMO-ligand binary complexes. Our findings shed light on the nature of the interaction between NEMO and poly-ubiquitin, suggesting that NEMO is differentially regulated by poly-ubiqutin chain length and that this regulation occurs via a modulation of the available equilibrium of conformational states, rather than gross structural change. This study therefore demonstrates the potential of REES as a powerful tool for tackling contemporary issues in structural biology and biophysics and elucidates novel information on the structure-function relationship of NEMO and key interaction partners. This article is protected by copyright. All rights reserved.

    Details

    Item Type Articles
    CreatorsCatici, D. A. M., Amos, H. E., Yang, Y., van den Elsen, J. M. H. and Pudney, C. R.
    DOI10.1111/febs.13724
    DepartmentsFaculty of Science > Biology & Biochemistry
    Research CentresCentre for Sustainable Chemical Technologies
    RefereedYes
    StatusPublished
    ID Code49844
    Additional InformationThis article is protected by copyright. All rights reserved.

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