Research

Ozone gas as a benign sterilization treatment for PLGA nanofibre scaffolds


Reference:

Rediguieri, C. F., de Jesus Andreoli Pinto, T., Bou-Chacra, N. A., Galante, R., Lima Barros de Araújo, G., do Nascimento Pedrosa, T., Maria-Engler, S. S., De Bank, P. A. and , P. D. B., 2016. Ozone gas as a benign sterilization treatment for PLGA nanofibre scaffolds. Tissue Engineering part C Methods, 22 (4), pp. 338-347.

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    Official URL:

    http://dx.doi.org/10.1089/ten.tec.2015.0298

    Abstract

    The use of electrospun nanofibres for tissue engineering and regenerative medicine applications is a growing trend as they provide improved support for cell proliferation and survival due, in part, to their morphology mimicking that of the extracellular matrix. Sterilization is a critical step in the fabrication process of implantable biomaterial scaffolds for clinical use, but many of the existing methods employed to date can negatively affect scaffold properties and performance. Poly(lactic-co-glycolic acid) (PLGA) has been widely used as a biodegradable polymer for 3D scaffolds, and can be significantly affected by current sterilization techniques. The aim of this study was to investigate pulsed ozone gas as an alternative method for sterilizing PLGA nanofibres. The morphology, mechanical properties, physicochemical properties, and response of cells to PLGA nanofibre scaffolds were assessed following different degrees of ozone gas sterilization. This treatment killed Geobacillus stearothermophilus spores, the most common biological indicator used for validation of sterilization processes. In addition, the method preserved all of the characteristics of non-sterilized PLGA nanofibres at all degrees of sterilization tested. These findings suggest that ozone gas can be applied as an alternative method for sterilizing electrospun PLGA nanofibre scaffolds without detrimental effects.

    Details

    Item Type Articles
    CreatorsRediguieri, C. F., de Jesus Andreoli Pinto, T., Bou-Chacra, N. A., Galante, R., Lima Barros de Araújo, G., do Nascimento Pedrosa, T., Maria-Engler, S. S., De Bank, P. A. and , P. D. B.
    DOI10.1089/ten.TEC.2015.0298
    Uncontrolled Keywordspolymeric scaffolds,nanofibres,sterilization,tissue engineering,regenerative medicine
    DepartmentsFaculty of Science > Pharmacy & Pharmacology
    Research CentresCentre for Regenerative Medicine
    RefereedYes
    StatusPublished
    ID Code49896

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