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Platelet lysate gel and endothelial progenitors stimulate microvascular network formation in vitro: tissue engineering implications


Reference:

Fortunato, T., Beltrami, C., Emanueli, C., De Bank, P. and Pula, G., 2016. Platelet lysate gel and endothelial progenitors stimulate microvascular network formation in vitro: tissue engineering implications. Scientific Reports, 6, 25326.

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    Official URL:

    http://dx.doi.org/10.1038/srep25326

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    Abstract

    Revascularisation is a key step for tissue regeneration and complete organ engineering. We describe the generation of human platelet lysate gel (hPLG), an extracellular matrix preparation from human platelets able to support the proliferation of endothelial colony forming cells (ECFCs) in 2D cultures and the formation of a complete microvascular network in vitro in 3D cultures. Existing extracellular matrix preparations require addition of high concentrations of recombinant growth factors and allow only limited formation of capillary-like structures. Additional advantages of our approach over existing extracellular matrices are the absence of any animal product in the composition hPLG and the possibility of obtaining hPLG from patients to generate homologous scaffolds for re-implantation. This discovery has the potential to accelerate the development of regenerative medicine applications based on implantation of microvascular networks expanded ex vivo or the generation of fully vascularised organs.

    Details

    Item Type Articles
    CreatorsFortunato, T., Beltrami, C., Emanueli, C., De Bank, P. and Pula, G.
    DOI10.1038/srep25326
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    URLURL Type
    http://dx.doi.org/10.1038/srep25326Free Full-text
    DepartmentsFaculty of Science > Pharmacy & Pharmacology
    Research CentresCentre for Regenerative Medicine
    RefereedYes
    StatusPublished
    ID Code50305

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