Research

Thermal stability, storage and release of proteins with tailored fit in silica


Reference:

Chen, Y.-C., Smith, T., Hicks, R. H., Doekhie, A., Koumanov, F., Wells, S. A., Edler, K. J., van den Elsen, J., Holman, G. D., Marchbank, K. J. and Sartbaeva, A., 2017. Thermal stability, storage and release of proteins with tailored fit in silica. Scientific Reports, 7, 46568.

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https://doi.org/10.1038/srep46568

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Abstract

Biological substances based on proteins, including vaccines, antibodies, and enzymes, typically degrade at room temperature over time due to denaturation, as proteins unfold with loss of secondary and tertiary structure. Their storage and distribution therefore relies on a "cold chain" of continuous refrigeration; this is costly and not always effective, as any break in the chain leads to rapid loss of effectiveness and potency. Efforts have been made to make vaccines thermally stable using treatments including freeze-drying (lyophilisation), biomineralisation, and encapsulation in sugar glass and organic polymers. Here for the first time we show that proteins can be enclosed in a deposited silica "cage", rendering them stable against denaturing thermal treatment and long-term ambient-temperature storage, and subsequently released into solution with their structure and function intact. This "ensilication" method produces a storable solid protein-loaded material without the need for desiccation or freeze-drying. Ensilication offers the prospect of a solution to the "cold chain" problem for biological materials, in particular for vaccines.

Details

Item Type Articles
CreatorsChen, Y.-C., Smith, T., Hicks, R. H., Doekhie, A., Koumanov, F., Wells, S. A., Edler, K. J., van den Elsen, J., Holman, G. D., Marchbank, K. J. and Sartbaeva, A.
DOI10.1038/srep46568
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URLURL Type
https://doi.org/10.1038/srep46568Free Full-text
Uncontrolled Keywordsjournal article
DepartmentsFaculty of Science > Chemistry
Faculty of Science > Biology & Biochemistry
Faculty of Humanities & Social Sciences > Health
Faculty of Engineering & Design > Chemical Engineering
Research CentresCentre for Sustainable Chemical Technologies
Centre for Nanoscience and Nanotechnology
RefereedYes
StatusPublished
ID Code55307

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