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Comparative Genomics of Campylobacter fetus from Reptiles and Mammals Reveals Divergent Evolution in Host-Associated Lineages


Reference:

Gilbert, M. J., Miller, W. G., Yee, E., Zomer, A. L., van der Graaf-van Bloois, L., Fitzgerald, C., Forbes, K. J., Méric, G., Sheppard, S. K., Wagenaar, J. A. and Duim, B., 2016. Comparative Genomics of Campylobacter fetus from Reptiles and Mammals Reveals Divergent Evolution in Host-Associated Lineages. Genome Biology and Evolution, 8 (6), pp. 2006-2019.

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Official URL:

https://doi.org/10.1093/gbe/evw146

Abstract

Campylobacter fetus currently comprises three recognized subspecies, which display distinct host association. Campylobacter fetus subsp. fetus and C fetus subsp. venerealis are both associated with endothermic mammals, primarily ruminants, whereas C fetus subsp. testudinum is primarily associated with ectothermic reptiles. Both C. fetus subsp. testudinum and C. fetus subsp. fetus have been associated with severe infections, often with a systemic component, in immunocompromised humans. To study the genetic factors associated with the distinct host dichotomy in C. fetus, whole-genome sequencing and comparison of mammal- and reptile-associated C fetus was performed. The genomes of C fetus subsp. testudinum isolated from either reptiles or humans were compared with elucidate the genetic factors associated with pathogenicity in humans. Genomic comparisons showed conservation of gene content and organization among C fetus subspecies, but a clear distinction between mammal- and reptile-associated C fetus was observed. Several genomic regions appeared to be subspecies specific, including a putative tricarballylate catabolism pathway, exclusively present in C fetus subsp. testudinum strains. Within C fetus subsp. testudinum, sapA, sapB, and sapAB type strains were observed. The recombinant locus iamABC (mlaFED) was exclusively associated with invasive C fetus subsp. testudinum strains isolated from humans. A phylogenetic reconstruction was consistent with divergent evolution in host-associated strains and the existence of a barrier to lateral gene transfer between mammal- and reptile-associated C fetus Overall, this study shows that reptile-associated C fetus subsp. testudinum is genetically divergent from mammal-associated C fetus subspecies.

Details

Item Type Articles
CreatorsGilbert, M. J., Miller, W. G., Yee, E., Zomer, A. L., van der Graaf-van Bloois, L., Fitzgerald, C., Forbes, K. J., Méric, G., Sheppard, S. K., Wagenaar, J. A. and Duim, B.
DOI10.1093/gbe/evw146
Uncontrolled Keywordsanimals,campylobacter infections,campylobacter fetus,evolution, molecular,gene transfer, horizontal,genetic variation,host-pathogen interactions,humans,mammals,phylogeny,reptiles,species specificity,journal article
DepartmentsFaculty of Science > Biology & Biochemistry
Research CentresMilner Centre for Evolution
RefereedYes
StatusPublished
ID Code56233
Additional Information© The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

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