Time-dependent inhibitors of trypanothione reductase. Analogues of the spermidine alkaloid lunarine and related natural products

Reference:

Hamilton, C. J., Saravanamuthu, A., Poupat, C., Fairlamb, A. H. and Eggleston, I. M., 2006. Time-dependent inhibitors of trypanothione reductase. Analogues of the spermidine alkaloid lunarine and related natural products. Bioorganic and Medicinal Chemistry, 14, pp. 2266-2278.

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Official URL:

http://dx.doi.org/10.1016/j.bmc.2005.11.004

Abstract

The macrocyclic spermidine alkaloid lunarine 1 from Lunaria biennis is a competitive, time-dependent inhibitor of the protozoan oxidoreductase trypanothione reductase (TryR), a promising target in drug design against tropical parasitic diseases. Various molecules related to 1 and the alkaloid itself have been synthesized in racemic form and evaluated against TryR in order to determine the key features of 1 that are associated with time-dependent inhibition. Kinetic data are consistent with an inactivation mechanism involving a conjugate addition of an active site cysteine residue onto the C-24–C-25 double bond of the tricyclic nucleus of 1. Comparison of data for synthetic (±)-1, the natural product, and other derivatives 7–10 from L. biennis confirms the importance of the unique structure of the tricyclic core as a motif for inhibitor design and reveals that the non-natural enantiomer may be a more suitable scaffold upon which thiophilic groups may be presented.

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