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Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates


Reference:

Pound, J. C., Green, D. W., Chaudhuri, J. B., Mann, S., Roach, H. I. and Oreffo, R. O. C., 2006. Strategies to Promote Chondrogenesis and Osteogenesis from Human Bone Marrow Cells and Articular Chondrocytes Encapsulated in Polysaccharide Templates. Tissue Engineering, 12 (10), pp. 2789-2799.

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Abstract

The aim of this study was to synthesize functional in vitro and in vivo 3-dimensional (3D) constructs using a mix of human mesenchymal populations and articular chondrocytes encapsulated in biomineralized polysaccharide templates. Single-cell-type populations or mixts. of both cell types were encapsulated in alginate/chitosan and cultured within a rotating-bioreactor, perfused bioreactor system, or static conditions for 28 days. Within single cell-type populations, type II collagen immunopos. cells were present within lacunae in rotating-bioreactor capsules, with an increased proportion of metabolically active cells compared with perfused and static constructs. Biochem. anal. indicated significantly increased ( p < 0.05) DNA and protein in rotating-bioreactor conditions compared with perfused or static. However, in coculture samples, DNA and protein was significantly increased in static cultures owing to the formation of large regions of partially mineralized osteoid. This osteoid was found only in static cultures and when the ratio of human bone marrow cells to chondrocytes was 2:1 or, to a lesser extent, 5:1 ratio capsules. S.c. implantation of capsules into immunocompromised mice also showed optimal osteoid formation when the ratio was 2:1. The current studies demonstrate the pivotal role of robust 3D biomimetic microenvironments and indicate the potential to harness the interactions between different cell types to create specific tissues. [on SciFinder (R)]

Details

Item Type Articles
CreatorsPound, J. C., Green, D. W., Chaudhuri, J. B., Mann, S., Roach, H. I. and Oreffo, R. O. C.
Uncontrolled Keywordschitosan proliferated and showed chondrogenesis well in rotating-bioreactor whereas coculture showed osteoid formation in static bioreactor and in mouse), bone (osteoid, chitosan proliferated and showed chondrogenesis indicated by type ii collagen immunopos. cell), chitosan proliferated and differentiated well in rotating-bioreactor whereas coculture showed osteoid formation in static bioreactor and in mouse), bone formation, bone marrow, unclassified), chondrocyte (articular, chitosan proliferated and showed chondrogenesis well in rotating-bioreactor whereas coculture showed osteoid formation in static bioreactor and in mouse), encapsulation, biol (biological study) (type i, bsu (biological study, chondrogenesis osteogenesis bioreactor articular chondrocyte differentiation, bioreactors, chitosan coculture showed osteogenesis by osteoid formation in static but not rotating or perfused bioreactor and in mouse), biol (biological study) (type ii, cartilage formation, collagens role, chitosan showed osteogenesis by osteoid formation in static bioreactor and in mouse as indicated by type i collagen immunopos. cell)
DepartmentsFaculty of Engineering & Design > Chemical Engineering
RefereedYes
StatusPublished
ID Code861

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