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A Comparison of Colorimetric and DNA Quantification Assays for the Assessment of Meniscal Fibrochondrocyte Proliferation in Microcarrier Culture


Reference:

Pabbruwe, M. B., Stewart, K. and Chaudhuri, J. B., 2005. A Comparison of Colorimetric and DNA Quantification Assays for the Assessment of Meniscal Fibrochondrocyte Proliferation in Microcarrier Culture. Biotechnology Letters, 27 (19), pp. 1451-1455.

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Abstract

We have developed and refined a rapid, reliable method for the evaluation of attachment and proliferation of ovine meniscal chondrocytes in microcarrier culture. Assays measuring both mitochondrial activity, using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium], and DNA synthesis with a PicoGreen assay were compared. The MTT assay was the most sensitive at lower cell concns. and enabled accurate assessment of cell proliferation over 14 day culture. [on SciFinder (R)]

Details

Item Type Articles
CreatorsPabbruwe, M. B., Stewart, K. and Chaudhuri, J. B.
Uncontrolled Keywordscarriers (microcarriers, animal tissue culture, anst (analytical study) (comparison of colorimetric and dna quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture), colorimetry mitochondria dna chondrocyte culture microcarrier mtt mts, comparison of colorimetric and dna quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture), chondrocyte (meniscal, cytodex 1, dna formation, colorimetry, mitochondria, ovis aries (comparison of colorimetric and dna quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture), 3, 2, dna role, ant (analyte), cell proliferation
DepartmentsFaculty of Engineering & Design > Chemical Engineering
RefereedYes
StatusPublished
ID Code903

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