A Comparison of Colorimetric and DNA Quantification Assays for the Assessment of Meniscal Fibrochondrocyte Proliferation in Microcarrier Culture
Reference:
Pabbruwe, M. B., Stewart, K. and Chaudhuri, J. B., 2005. A Comparison of Colorimetric and DNA Quantification Assays for the Assessment of Meniscal Fibrochondrocyte Proliferation in Microcarrier Culture. Biotechnology Letters, 27 (19), pp. 1451-1455.
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Abstract
We have developed and refined a rapid, reliable method for the evaluation of attachment and proliferation of ovine meniscal chondrocytes in microcarrier culture. Assays measuring both mitochondrial activity, using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium], and DNA synthesis with a PicoGreen assay were compared. The MTT assay was the most sensitive at lower cell concns. and enabled accurate assessment of cell proliferation over 14 day culture. [on SciFinder (R)]
Details
| Item Type | Articles |
| Creators | Pabbruwe, M. B., Stewart, K. and Chaudhuri, J. B. |
| Uncontrolled Keywords | carriers (microcarriers, animal tissue culture, anst (analytical study) (comparison of colorimetric and dna quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture), colorimetry mitochondria dna chondrocyte culture microcarrier mtt mts, comparison of colorimetric and dna quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture), chondrocyte (meniscal, cytodex 1, dna formation, colorimetry, mitochondria, ovis aries (comparison of colorimetric and dna quantification assays for assessment of meniscal fibrochondrocyte proliferation in microcarrier culture), 3, 2, dna role, ant (analyte), cell proliferation |
| Departments | Faculty of Engineering & Design > Chemical Engineering |
| Refereed | Yes |
| Status | Published |
| ID Code | 903 |
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