Histone Tail-independent Chromatin Binding Activity of Recombinant Cohesin Holocomplex
Kagansky, A., Freeman, L., Lukyanov, D. and Strunnikov, A., 2004. Histone Tail-independent Chromatin Binding Activity of Recombinant Cohesin Holocomplex. Journal of Biological Chemistry, 279 (5), pp. 3382-3388.
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Cohesin, an SMC (structural maintenance of chromosomes) protein-contg. complex, governs several important aspects of chromatin dynamics, including the essential chromosomal process of sister chromatid cohesion. The exact mechanism by which cohesin achieves the bridging of sister chromatids is not known. To elucidate this mechanism, we reconstituted a recombinant cohesin complex and investigated its binding to DNA fragments corresponding to natural chromosomal sites with high and low cohesin occupancy in vivo. Cohesin displayed uniform but nonspecific binding activity with all DNA fragments tested. Interestingly, DNA fragments with high occupancy by cohesin in vivo showed strong nucleosome positioning in vitro. We therefore utilized a defined model chromatin fragment (purified reconstituted dinucleosome) as a substrate to analyze cohesin interaction with chromatin. The four-subunit cohesin holocomplex showed a distinct chromatin binding activity in vitro, whereas the Smc1p-Smc3p dimer was unable to bind chromatin. Histone tails and ATP are dispensable for cohesin binding to chromatin in this reaction. A model for cohesin assocn. with chromatin is proposed. [on SciFinder (R)]
|Creators||Kagansky, A., Freeman, L., Lukyanov, D. and Strunnikov, A.|
|Uncontrolled Keywords||biol (biological study) (complexes,bsu (biological study,chromatin,formation of stable complex between cohesin and chromatin suggests chromatin structure dets. configuration of sister chromatid cohesion site),cohesin holocomplex interaction chromatin sister chromatid cohesion,molecular association (recombinant yeast cohesin holocomplex exhibits histone tail-independent chromatin binding activity),proteins role,unclassified),cohesin,recombinant yeast cohesin holocomplex exhibits histone tail-independent chromatin binding activity),chromatid (sister|
|Departments||Faculty of Engineering & Design > Chemical Engineering|
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