Porcine collagen crosslinking, degradation and its capability for fibroblast adhesion and proliferation
Jarman-Smith, M. L., Bodamyali, T., Stevens, C., Howell, J. A., Horrocks, M. and Chaudhuri, J. B., 2004. Porcine collagen crosslinking, degradation and its capability for fibroblast adhesion and proliferation. Journal of Materials Science: Materials in Medicine, 15 (8), pp. 925-932.
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Porcine dermal collagen permanently crosslinked with hexamethylene diisocyanate was investigated for its suitability as a dermal tissue engineering matrix. It was found that the chem. crosslinked collagen had far fewer free lysine groups per collagen mol. than did the uncrosslinked matrix. The ability of the matrix to support human primary fibroblast outgrowth from explants was compared for matrixes that had been presoaked in various solns., including fibroblast media, cysteine and phosphate buffered saline (PBS). It was found that superior cell outgrowth was obtained after soaking with fibroblast media and PBS. The fibroblast attachment properties of the matrix were compared against tissue culture plastic and PET. The collagen matrix showed the least amt. of cell retention compared to the other to matrixes, however, the general trends were similar for all three scaffolds. Longer term cultures on the collagen showed fibroblasts covering the matrix stacking up on each other and bridging natural hair follicles. However, it was also obsd. that the fibroblasts were not able to penetrate into the matrix structure. This was believed to result from the chem. crosslinking, as shown by the resistance of the matrix to degrdn. by collagenases. [on SciFinder (R)]
|Creators||Jarman-Smith, M. L., Bodamyali, T., Stevens, C., Howell, J. A., Horrocks, M. and Chaudhuri, J. B.|
|Uncontrolled Keywords||animal tissue culture, crosslinking, porcine collagen crosslinking, degrdn. and its capability for fibroblast adhesion and proliferation), unclassified), biol (biological study), adhesion, proc (process), uses (uses) (type i, bsu (biological study, thu (therapeutic use), porcine collagen crosslinking degrdn fibroblast adhesion proliferation, cell proliferation, pep (physical, cps (chemical process), collagens role, fibroblast (porcine collagen crosslinking, engineering or chemical process)|
|Departments||Faculty of Engineering & Design > Chemical Engineering|
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